File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s014.ogv

The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s014.ogv(Ogg Theora video file, length 34 s, 266 × 266 pixels, 186 kbps, file size: 771 KB)

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English: (Related to Fig. 4B). Dynamics of in vivo GFP-Lc3 recruitment to DsRed-Shigella. DsRed-Shigella escape to the cytosol and induce septin cages and autophagy in zebrafish larvae in vivo. GFP-Lc3 zebrafish larvae were infected subcutaneously with DsRed-Shigella and followed by confocal fluorescence microscopy. Single GFP-Lc3 positive leukocytes accumulating GFP-lc3 around Shigella are tracked by arrows; single GFP-Lc3 positive leukocytes having engulfed the bacteria without GFP-Lc3 accumulation are highlighted by white circles. The movie starts 3h10min pi (T = 0 on the movie) and shows 2h50min acquisition. Time lapse every 1 min. The maximum intensity projection from 7 planes every 2 µm is shown. Scale bar, 20 µm.
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Source Video S8 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221.
Author Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E
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current20:37, 14 September 201334 s, 266 × 266 (771 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 67 kbps Completed 23:08, 21 October 2018 6.0 s
Streaming 240p (VP9) 67 kbps Completed 18:24, 17 December 2023 1.0 s
WebM 360P Not ready Unknown status
Streaming 144p (MJPEG) 195 kbps Completed 14:36, 9 November 2023 1.0 s

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