File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s014.ogv
The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s014.ogv (Ogg Theora video file, length 34 s, 266 × 266 pixels, 186 kbps, file size: 771 KB)
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editDescriptionThe-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s014.ogv |
English: (Related to Fig. 4B). Dynamics of in vivo GFP-Lc3 recruitment to DsRed-Shigella. DsRed-Shigella escape to the cytosol and induce septin cages and autophagy in zebrafish larvae in vivo. GFP-Lc3 zebrafish larvae were infected subcutaneously with DsRed-Shigella and followed by confocal fluorescence microscopy. Single GFP-Lc3 positive leukocytes accumulating GFP-lc3 around Shigella are tracked by arrows; single GFP-Lc3 positive leukocytes having engulfed the bacteria without GFP-Lc3 accumulation are highlighted by white circles. The movie starts 3h10min pi (T = 0 on the movie) and shows 2h50min acquisition. Time lapse every 1 min. The maximum intensity projection from 7 planes every 2 µm is shown. Scale bar, 20 µm. |
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Source | Video S8 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221. | ||
Author | Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E | ||
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current | 20:37, 14 September 2013 | 34 s, 266 × 266 (771 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | (Related to Fig. 4B). Dynamics of in vivo GFP-Lc3 recruitment to DsRed-Shigella. DsRed-Shigella escape to the cytosol and induce septin cages and autophagy in zebrafish larvae in vivo. GFP-Lc3 zebrafish larvae were infected subcutaneously with DsRed-Shigella and followed by confocal fluorescence microscopy. Single GFP-Lc3 positive leukocytes accumulating GFP-lc3 around Shigella are tracked by arrows; single GFP-Lc3 positive leukocytes having engulfed the bacteria without GFP-Lc3 accumulation are highlighted by white circles. The movie starts 3h10min pi (T? |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |