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File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s016.ogv

The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s016.ogv ‎(Ogg Theora video file, length 6.8 s, 250 × 250 pixels, 306 kbps, file size: 254 KB)

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English: (Related to Fig. S4B). Dynamics of in vivo GFP-Lc3 recruitment to DsRed-M. marinum. M. marinum escape to the cytosol and induce septin cages and autophagy in zebrafish larvae in vivo. GFP-Lc3 zebrafish larvae were infected subcutaneously with DsRed-M. marinum and followed by confocal fluorescence microscopy. Shown here is an example of an GFP-Lc3 positive leukocyte (arrow) controlling M. marinum and progressively accumulating GFP-Lc3 to the internalized bacteria. In the same field, note two GFP-Lc3 positive leukocytes (white circles) that do not accumulate GFP-Lc3 to the internalized bacteria, and that presumably do not control intracellular M. marinum proliferation. The movie starts 1h10mpi (T = 0 on the movie) and ends 2h30mpi. Time lapse every 2′30″. The maximum intensity projection from 5 planes every 2 µm is shown. Scale bar, 20 µm.
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Source Video S10 from Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E (2013). "The Zebrafish as a New Model for the In Vivo Study of Shigella flexneri Interaction with Phagocytes and Bacterial Autophagy". PLOS Pathogens. DOI:10.1371/journal.ppat.1003588. PMC: 3764221.
Author Mostowy S, Boucontet L, Mazon Moya M, Sirianni A, Boudinot P, Hollinshead M, Cossart P, Herbomel P, Levraud J, Colucci-Guyon E
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current20:37, 14 September 20136.8 s, 250 × 250 (254 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 106 kbps Completed 23:09, 21 October 2018 3.0 s
Streaming 240p (VP9) 107 kbps Completed 18:24, 17 December 2023 1.0 s
WebM 360P 149 kbps Completed 16:02, 2 December 2023 0.0 s
Streaming 144p (MJPEG) 249 kbps Completed 14:37, 9 November 2023 1.0 s

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