English: Supplementary Movie 9 KlpA exhibits opposite directional preference inside and outside the antiparallel microtubule overlap. Movies showing that GFP-KlpA molecules (green) move preferentially toward the plus end of the track microtubule (blue) outside the antiparallel overlap and collectively transport the cargo microtubule (red) toward the minus end of the track microtubule. The cargo and track microtubule were both polarity-marked with a bright plus end, and fluorescently labeled with TMR and Hilyte 647 respectively. From Top to Bottom: the track microtubule channel (the movie was made from a single snapshot of the track microtubule); the GFP-KlpA channel; the cargo microtubule channel; and the overlay of the GFP-KlpA and cargo microtubule channels. Movie corresponds to Fig. 4b. Arrowhead indicates the plus end of the track microtubule used for generating the kymograph in Fig. 4b.