English: Supplementary Movie 10 KlpA exhibits opposite directional preference inside and outside the parallel microtubule overlap. Movies showing that GFP-KlpA molecules (green) move preferentially toward the plus end of the track microtubule (blue) outside the parallel overlap area but preferentially move to accumulate at the minus end of the cargo microtubule inside the parallel overlap area. The cargo and track microtubule were both polarity-marked with a bright plus end, and fluorescently labeled with TMR and Hilyte 647 respectively. Top: the track microtubule channel, and the movie was made from a single snapshot of the track microtubule; Second from the top: the GFP-KlpA channel; Third from the top: the cargo microtubule channel; Bottom: Overlay of the GFP-KlpA and cargo microtubule channels. Movie corresponds to Fig. 4c. Arrowhead indicates the plus end of the track microtubule used for generating the kymograph in Fig. 4c.