File:Time-Lapse-Imaging-of-the-Dynamics-of-CNS-Glial-Axonal-Interactions-In-Vitro-and-Ex-Vivo-pone.0030775.s002.ogv
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Time-Lapse-Imaging-of-the-Dynamics-of-CNS-Glial-Axonal-Interactions-In-Vitro-and-Ex-Vivo-pone.0030775.s002.ogv (Ogg Theora video file, length 28 s, 696 × 520 pixels, 702 kbps, file size: 2.31 MB)
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editDescriptionTime-Lapse-Imaging-of-the-Dynamics-of-CNS-Glial-Axonal-Interactions-In-Vitro-and-Ex-Vivo-pone.0030775.s002.ogv |
English: In vitro time-lapse imaging of fluorescently labelled cells in association with neurites. Myelinating cultures generated from a mix of wild type/beta-actin (cyto GFP) mice were visualised using time-lapse microscopy (Nikon TE2000 (60×, 0.75NA) over 16 hr in 5 min intervals on DIV 27 after the addition of wild type neurospheres previously infected with lentivirus carrying dsRed/GFP gene and addition of cyto-GFP cells. A strongly green fluorescent cell can be seen to dynamically interact with a bundle of neurties. A red labelled cell can be seen to be engulfed by a cell resembling microglia. |
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Source | Video S2 from Ioannidou K, Anderson K, Strachan D, Edgar J, Barnett S (2012). "Time-Lapse Imaging of the Dynamics of CNS Glial-Axonal Interactions In Vitro and Ex Vivo". PLOS ONE. DOI:10.1371/journal.pone.0030775. PMID 22303455. PMC: 3267745. | ||
Author | Ioannidou K, Anderson K, Strachan D, Edgar J, Barnett S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 17:54, 14 November 2012 | 28 s, 696 × 520 (2.31 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ioannidou K, Anderson K, Strachan D, Edgar J, Barnett S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |