File:Tissue processing - Tissue sections on slides are stained on an automated stainer.jpg
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DescriptionTissue processing - Tissue sections on slides are stained on an automated stainer.jpg |
Tissue sections on slides are stained using hematoxylin and eosin on an automated stainer. This is the routine "H&E" stain, which is used on almost all specimens. Hematoxylin, which is blue or purple, stains mostly nuclei, while the pink to orange eosin stains cytoplasm, nucleoli, and extracellular substances like collagen. After staining, the processor takes the slides through a dehydrating solvent, usually alcohol or acetone, and then into a "clearing agent" either xylene or a citrus-smelling substance called Americlear. Sometimes it is necessary to supplement the H&E with "special stains" or "immunostains" |
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Date | |||
Source | originally posted to Flickr as Tissue Processing 12.jpg | ||
Author | Ed Uthman | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution-Share Alike 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:33, 8 November 2008 | 2,848 × 4,288 (5.21 MB) | Flickr upload bot (talk | contribs) | Uploaded from http://flickr.com/photo/78147607@N00/275494796 using Flickr upload bot |
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Camera manufacturer | NIKON CORPORATION |
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Camera model | NIKON D2X |
Exposure time | 1/60 sec (0.016666666666667) |
F-number | f/8 |
ISO speed rating | 400 |
Date and time of data generation | 09:04, 20 October 2006 |
Lens focal length | 32 mm |
Orientation | Normal |
Horizontal resolution | 300 dpi |
Vertical resolution | 300 dpi |
Software used | Adobe Photoshop 7.0 |
File change date and time | 13:03, 21 October 2006 |
Y and C positioning | Co-sited |
Exposure Program | Aperture priority |
Exif version | 2.21 |
Date and time of digitizing | 09:04, 20 October 2006 |
Image compression mode | 4 |
APEX exposure bias | 0 |
Maximum land aperture | 3 APEX (f/2.83) |
Metering mode | Pattern |
Light source | Unknown |
Flash | Flash fired, strobe return light detected |
DateTime subseconds | 08 |
DateTimeOriginal subseconds | 08 |
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Color space | sRGB |
Sensing method | One-chip color area sensor |
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