English: Fluorescence movie montage for strain λWT corresponding to a typical, long movie. Single-color images for TetR-EYFP (top left) and LacI-mCherry (top right) data have intensities scaled linearly from the lowest to the highest pixel values in the first image in each time series. Before creating the overlay images (bottom), single-color images were background subtracted and bandpass filtered using the program ImageJ [91]. The overlay images are scaled to be twice as large as the single-color images. Scale bars correspond 4 µm in the small, single-color images and 2 µm in the overlay image. Twelve consecutive image frames are shown in real time (10 frames per second); the movie is looped 5 times.