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File:Visualization-of-Content-Release-from-Cell-Surface-Attached-Single-HIV-1-Particles-Carrying-an-pone.0148944.s001.ogv

Visualization-of-Content-Release-from-Cell-Surface-Attached-Single-HIV-1-Particles-Carrying-an-pone.0148944.s001.ogv ‎(Ogg Theora video file, length 15 s, 652 × 396 pixels, 11.45 Mbps, file size: 20.54 MB)

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English: Extra-viral pH-sensor blinks as HIV-1 pseudoparticle shuttles between neutral and acidic cellular compartments. HXB2 pseudoparticles labeled with releasable content marker Gag-imCherry (red) and extra-viral pH sensor EcpH-ICAM (green) were pre-bound in the cold to CV1/CD4/CXCR4 cells and imaged for 1 hour at 37°C. Particle trafficking to mildly acidic cellular compartment completely quenches fluorescence from the EcpH-ICAM label. ~0.8% of particles exhibited dequenching of EcpH-ICAM following quenching, suggesting trafficking of the particle back to a neutral compartment or the cell surface.
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Source S1 Movie from Sood C, Marin M, Mason C, Melikyan G (2016). "Visualization of Content Release from Cell Surface-Attached Single HIV-1 Particles Carrying an Extra-Viral Fluorescent pH-Sensor". PLOS ONE. DOI:10.1371/journal.pone.0148944. PMID 26863211. PMC: 4749635.
Author Sood C, Marin M, Mason C, Melikyan G
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current06:39, 29 February 201615 s, 652 × 396 (20.54 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 642 kbps Completed 18:56, 25 October 2018 28 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 316 kbps Completed 18:56, 25 October 2018 25 s
Streaming 240p (VP9) 316 kbps Completed 00:50, 22 December 2023 1.0 s
WebM 360P 592 kbps Completed 06:40, 29 February 2016 22 s
Streaming 144p (MJPEG) 862 kbps Completed 03:02, 18 November 2023 1.0 s

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