File:Visualizing-Vpr-Induced-G2-Arrest-and-Apoptosis-pone.0086840.s013.ogv
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Visualizing-Vpr-Induced-G2-Arrest-and-Apoptosis-pone.0086840.s013.ogv (Ogg Theora video file, length 32 s, 680 × 512 pixels, 107 kbps, file size: 414 KB)
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editDescriptionVisualizing-Vpr-Induced-G2-Arrest-and-Apoptosis-pone.0086840.s013.ogv |
English: Time-lapse imaging of the effect of continuous expression of DD-Vpr in HeLa/Fucci2 cells. HeLa/Fucci2 cells were transfected with pME/DD-Vpr-IRES-ECFP. Twenty-four hours after transfection, the cells were treated with 500 nM Shield1 for 23 h, at which point the cells were washed to remove Shield1 and further cultured in the presence of 500 nM Shield1 for 1 h. ECFP-expressing cells were observed with LCV110 Imaging System at 15 min intervals for 72 h. |
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Source | Video S7 from Murakami T, Aida Y (2014). "Visualizing Vpr-Induced G2 Arrest and Apoptosis". PLOS ONE. DOI:10.1371/journal.pone.0086840. PMC: 3899331. | ||
Author | Murakami T, Aida Y | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 22:53, 25 January 2014 | 32 s, 680 × 512 (414 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Murakami T, Aida Y |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Time-lapse imaging of the effect of continuous expression of DD-Vpr in HeLa/Fucci2 cells. HeLa/Fucci2 cells were transfected with pME/DD-Vpr-IRES-ECFP. Twenty-four hours after transfection, the cells were treated with 500 nM Shield1 for 23 h, at which point the cells were washed to remove Shield1 and further cultured in the presence of 500 nM Shield1 for 1 h. ECFP-expressing cells were observed with LCV110 Imaging System at 15 min intervals for 72 h. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |